FIRST TIME EXPERIENCE OF APPLICATION OF IMMUNE HISTOCHEMICAL TESTS OF THE RETROPERITONEAL TISSUE IN THE EARLY PHASE OF ACUTE NECROTIZING PANCREATITIS
Abstract
Background. Treatment of patients with acute necrotizing pancreatitis (SNP) against the background of an evergrowing incidence and stably high mortality in severe forms of the disease has remained an unresolved task in urgent abdominal surgery.
Objective - to identify the capabilities of immune histochemical tests in the study of early morphological changes in retroperitoneal tissue of patients with acute necrotizing pancreatitis.
Materials and methods. The article presents the results of immune histochemical studies of retroperitoneal tissue biopsy samples of patients with acute necrotizing pancreatitis, taken in the early phase of the disease (within first 72 hours), with antibodies to CD 34, Cyclooxygenase 2 (COX2), C1q Complement (С1q), C3c Complement (C3c), Immunoglobulin A (IgA), Immunoglobulin G (IgG), Immunoglobulin M (IgM), Interleukin 18 (IL 18), Fibrinogen, Transforming growth factor beta 1 (TGF-β 1), Vascular Endothelial Growth Factor (VEGF).
Results. It has been proved that in case of acute pancreatitis development in retroperitoneal space tissues, one can observe the activation of cyclooxygenase 2, accumulation of exudate that contains the components of C1q and C3c complements, as well as A, G and M immunoglobulins, proinflammatory cytokine interleukin 18, fibrinogen, and also growth factors – TGF-β 1 and VEGF.
Conclusion. The obtained results indicate that in acute pancreatitis, immune histochemical methods can be successfully used to detect immune and inflammatory biomarkers.
References
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