THE METABOLISM OF GLUCOSE AS THE MAIN ENERGY SUBSTRATE OF HEPATOCYTES IN DIFFERENT METHODS OF LIVER PRESERVATION
Abstract
Background. Maintaining energy balance in hepatocytes is an important aspect of liver graft preservation. Objective – to conduct a comparative study of the effects of static cold storage and hypothermic oxygenated machine perfusion of liver grafts on the metabolism of glucose as the main energy substrate of hepatocytes. Material and methods. In this prospective case-control study (with marginal liver grafts splitting into two halves) we conducted a comparative analysis of the effects of static cold preservation and hypothermic oxygenated machine perfusion of liver transplants on the metabolism of glucose as the main energy substrate of liver cells using biochemical, morphological and immunohistochemical methods. Results. It has been shown that the use of hypothermic oxygenated machine perfusion is characterized by significantly lower values of the ratio of lactate to glucose in the effluent after 2 and 4 hours of perfusion and significantly lower values of the expression of glucose transporter molecules GLUT1 on hepatocyte membranes in comparison with static cold preservation. Conclusions. The use of hypothermic oxygenated machine perfusion even in marginal grafts allows to restore the respiratory chain in mitochondria and aerobic glycolysis enzymes of liver cells.References
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