PATHOMORPHOLOGICAL CHANGES ASSESSMENT IN EXPERIMENTAL DESTRUCTIVE PANCREATITIS IN RATS
Abstract
Background. Acute pancreatitis remains to be a challenge in emergency abdominal surgery. Severe forms of the disease have a number of complications associated with high mortality. There is a need for a thorough study of the pathogenesis of acute pancreatitis and its adequate pathomorphological assessment, first of all, in an experiment for the purpose of testing new therapeutic approaches. Objective. To assess pathomorphological changes in the pancreas and its surrounding tissues in experimental acute destructive pancreatitis (ADP) and to identify 4 categories of changes most specific for destructive pancreatitis. Material and methods. The experiment was performed on 54 rats. A special table was developed that includes 4 categories of pathomorphological changes and parameter assessment tool based on the visual analogue principle (VAP). Further statistical processing of data between the studied groups was performed. Results. Control group 1 is characterized by the absence of mortality and minimal pathomorphological changes, reflecting the response of the pancreas to the surgery. Group 2 is characterized by minimal mortality (16.67%), moderately increased indexes of necrosis, of circulatory disorders, of inflammation (up to the fourth day) as well as that of repair (by the eighth day), these parameters corresponding to ADP of moderate severity. In group 3, there is a high mortality rate (66.66%), a high index of necrosis with slight indexes of circulatory disorders and of inflammation (on the fourth day), as well as a low repair index (on the eighth day). This group is characterized by severe ADP. Conclusion. Administration of 10% detergent solution results in ADP of moderate severity. Administration of a 20% detergent solution causes severe ADP. The proposed table of pathomorphological assessment alongside with the mortality rate allow us to assess the severity of experimental acute destructive pancreatitis in rats.
References
Ibadov RA, Abdullajanov BR, Ibragimov SH, Nishanov MF. Tjazhelyj ostryj pankreatit. Vybor lechebnoj taktiki [Severe acute pancreatitis. Choice of treatment tactics]. Vestnik Nacionalnogo mediko-hirurgicheskogo Centra im. N.I. Pirogova [Bulletin of Pirogov National Medical and Surgical Center]. 2020;15(4):29-33. https://doi.org/10.25881/bpnmsc.2020.29.23.006. https://elibrary.ru/hgcxtt. (Russian).
Syshikov DS. Taktika hirurgicheskogo lechenija bolnyh s ostrym pankreatitom [Surgical treatment tactics for patients with acute pancreatitis]. Izvestija Rossijskoj voenno-medicinskoj akademii [Russian Military Medical Academy Reports]. 2020;39(Suppl 1):255-257. https://doi.org/10.17816/rmmar43448. (Russian).
Leonard-Murali S, Lezotte J, Kalu R, Blyden DJ, Patton JH, Johnson JL, Gupta AH. Necrotizing pancreatitis: A review for the acute care surgeon. Am J Surg. 2021;221(5):927-934. https://doi.org/10.1016/j.amjsurg.2020.08.027.
Lee DW, Cho CM. Predicting Severity of Acute Pancreatitis. Medicina (Kaunas). 2022;11(58):787. https://doi.org/10.3390/medicina58060787.
Gukovskaya AS, Gukovsky I, Algül H, Habtezion A. Autophagy, Inflammation, and Immune Dysfunction in the Pathogenesis of Pancreatitis. Gastroenterology. 2017;153(5):1212-1226. https://doi.org/10.1053/j.gastro.2017.08.071.
Imaeva AK, Mustafin TI, Sharafutdinova LA. Patomorfologicheskaja harakteristika strukturnyh i funkcionalnyh izmenenij podzheludochnoj zhelezy i parapankreaticheskoj zhirovoj tkani pri ostrom destruktivnom pankreatite. In: Chirskij VS, editor. Aktualnye voprosy patologicheskoj anatomii v mirnoe i voennoe vremya. Sbornik nauchnyh trudov Vserossijskoj konferencii; 2022 Apr. 22, Sankt-Peterburg. Sankt-Peterburg: BSMU; 2022. p. 25-30. edn: HDTAPI. (Russian).
Silva-Vaz P, Abrantes AM, Castelo-Branco M, Gouveia A, Botelho MF, Tralhão JG. Murine Models of Acute Pancreatitis: A Critical Appraisal of Clinical Relevance. Int J Mol Sci. 2019;20(11):2794. https://doi.org/10.3390/ijms20112794.
Shalimov SA, Radzihovskij AP, Kejsevich LV. Rukovodstvo po eksperimentalnoj hirurgii. Moskva: Medicina; 1989. Ch. 10, Modelirovanie zabolevanij organov pishhevarenija; p. 169-207. (Russian).
Koleshko SV, Lis RE. Modelirovanie patologicheskih processov v podzheludochnoj zheleze, maksimalno priblizhennyh k klinicheskoj praktike [Modeling of pathological processes in the pancreas, as close as possible to clinical practice]. Zhurnal Grodnenskogo gosudarstvennogo medicinskogo universiteta [Journal of the Grodno State Medical University]. 2009;2(26):159-162. EDN: QAUUDF. (Russian).
Wiley MB, Mehrotra K, Bauer J, Yazici C, Bialkowska AB, Jung B. Acute Pancreatitis: Current Clinical Approaches, Molecular Pathophysiology, and Potential Therapeutics. Pancreas. 2023;52(6):e335-e343. https://doi.org/10.1097/MPA.0000000000002259.
Guljanc ES, Lukash NA, Tkacheva TN, Surgutanova TA, Kalmykova JuA, inventors; Rostov Medical Institute, assignee. Metod modelirovanija pankreatita. SU patent 1327152. 1987 Jul 30. (Russian).
Danko K, Lukasheva E, Zhukov VA, Zgoda V, Frolov A. Detergent-Assisted Protein Digestion-On the Way to Avoid the Key Bottleneck of Shotgun Bottom-Up Proteomics. Int J Mol Sci. 2022;23(22):13903. https://doi.org/10.3390/ijms232213903.
Leontev VN, Ahramovich TI. Biohimija. Laboratornyj praktikum. Minsk: BGTU; 2008. 214 р. (Russian).
1.png)
