TRYPSIN-LIKE ACTIVITY OF BLOOD SERUM AS A MARKER OF CHRONIC LIVER DISEASES
Abstract
Background. The search for non-invasive criteria for the diagnosis of liver cirrhosis remains an urgent task at present. The level of trypsin-like activity may become an additional criterion for diagnosing this disease.
Objective. To assess the possibility of using trypsin-like activity for differential diagnostics of chronic hepatitis and liver cirrhosis.
Materials and methods. Trypsin-like activity of blood serum was evaluated in 60 patients with liver cirrhosis, 20 patients with chronic hepatitis and 25 donors from a blood transfusion station using modified Erlanger method.
Results. As a result of the study, a significant increase in trypsin-like serum activity in the group of patients with chronic hepatitis (p <0.001) and a decrease in those with liver cirrhosis (p <0.001) was found in comparison with donors. We established correlations of trypsin-like activity with clinico-laboratory parameters of the patients: the presence of portal hypertension, severity of ascites, splenomegaly, stage and degree of disease compensation, erythrocyte sedimentation rate, monocyte count, serum protein and glucose level, alkaline phosphatase activity, hemostasis system parameters, concentration of protein in the urine, the number of erythrocytes and white blood cells in the urine. In addition, the relationship with the Child-Pugh class of gravity and the de Ritis coefficient was revealed.
Conclusion. The level of trypsin-like activity can be used as a differential diagnostic criterion between cirrhosis and chronic hepatitis. The decrease in the level of BAPNA-amidase activity can be used as a marker of hepatic-cell insufficiency and liver fibrosis.
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