ULTRASTRUCTURAL CHANGES IN THE RENAL PROXIMAL TUBULE EPITHELIOCYTES IN THE DYNAMICS OF THE SUPRADUODENAL OBSTRUCTIVE JAUNDICE
Abstract
Background. Obstructive jaundice is accompanied by the development of endogenous intoxication, renal failure and hepatorenal syndrome, resulting in decompensation in the body.
Objective – to estimate the ultrastructural changes developing in the renal proximal tubule epithelial cells of rats in dynamics of experimental supraduodenal obstructive jaundice.
Materials and methods. Experimental supraduodenal obstructive jaundice (duration: 3 and 10 days) was simulated in rats by bandaging the common bile duct in its supraduodenal part. Sham operated animals were used as a control group. The concentration of common bile acids was determined by enzyme-colorimetric method in blood serum and urine of experimental and control rats. The electron microscope JEM-100 CX II of "JROL" company (Japan) was used for electron microscopic studies of rat kidneys.
Results. During 10 days of the experiment on the background of endotoxemia caused by marked cholatemia and cholaturia jaundiced animals have been developing severe ultrastructural changes in the renal proximal tubule epithelial cells, accompanied by disorganization of microvilli of the brush border, acute oedema of the cytoplasm with electron lucent zones appearing in it, accumulation of lysosomes containing matrix of heterogeneous density, and damage to mitochondria.
Conclusion. The observed severe ultrastructural changes in the tubular apparatus of nephrons result in the development of hepatorenal syndrome and multiple organ failure, the degree of severity of the latter depending on both the duration of supraduodenal cholestasis and cholatemia-induced endogenous intoxication.
References
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