MORPHOFUNCTIONAL CHARACTERISTICS OF MAST CELLS OF THE SMALL INTESTINE MUCOSA IN 15- AND 45-DAY RATS BORN TO MOTHERS WITH CHOLESTASIS AND SUBJECTED TO URSODEOXYCHOLIC ACID
Abstract
Background. Mast cells participate in any adaptive reactions and pathological processes of the body. They are found in large numbers in the organs of digestion, respiration, urinary system and fasciae.
Objective. To study the morphofunctional properties of mast cells in the small intestine of rats born to mothers with endogenous intoxication due to cholestasis and subjected to UDCA.
Materials and methods. The experiment was performed on 68 rats aged 15 and 45 days. Histological and histochemical methods were used, followed by morphometric and statistical analysis.
Results. Experimental subhepatic cholestasis, modeled on the 17th day of pregnancy lead to a significant increase not only in the total number of mast cells but also in the number of moderately and completely degranulated forms of these cells in the small intestine mucosa of 45-day-old rats.
Conclusion. Administration of UDCA at a dose of 50 mg/kg to pregnant female rats from the moment of modeling in them cholestasis and within 7 days after delivery, largely neutralizes in their offspring the revealed changes in mast cells.
References
1. Kocjuba AE, Chertok VM, Kocjuba EP, Babich EV. Osobennosti citohimii tuchnyh kletok v nekotoryh organah krysy [Features of the cytochemistry of mast cells in some organs of the rat]. Citologija. 2008;50(12):1023-1029. (Russian).
2. Galli SJ, Kalesnikoff J, Grimbaldeston MA, Piliponsky AM, Williams CM, Tsai M. Mast cells as “tunable” effector et immunoregulatory cells : recent advances. Annu. Rev. Immunol. 2005;23:749-786.
3. Zatolokina MA. Morfologija intramural’nogo nervnogo apparata, jendokrinnyh i tuchnyh kletok dvenadcatiperstnoj kishki pri hronicheskih narushenijah ejo prohodimosti [Morphology of the intramural nervous system, endocrine and mast cells of the duodenum with chronic violations of its patency]. Fundamentalnye issledovanija. 2004;2:132-133. (Russian).
4. Kondrashevskaja MV. Tuchnye kletki i geparin – kljuchevye zvenja v adaptivnyh i patologicheskih processah [Mast cells and heparin are key links in adaptive and pathological processes]. Vestnik Rossijskoj Akademii Medicinskih Nauk. 2010;6:49-54. (Russian).
5. Kondrashevskaja MV, Nikolskaja KA. Anksioliticheskoe i antinociceptivnoe dejstvie vysokomolekuljarnogo geparina na krys linii Vistar [Anxiolytic and antinociceptive action of high molecular weight heparin in Wistar rats]. Psihofarmakologija i biologicheskaja narkologija. 2002;3-4:407-408. (Russian).
6. Zajko NN, Byc JuV, Ataman AV. Patologicheskaja fiziologija [Pathological physiology]. 3th ed. Zajko NN, Byc JuV, editors. Moscow: MEDpress-inform; 2006. 640 p. (Russian).
7. Galli SJ, Tsai M. Mast cells in allergy and infection : versatile effector and regulatory cells in innate and adaptive immunity. Eur. J. Immunol. 2010;40(7):1843-51.
8. Hepworth MR, Daniłowicz-Luebert E, Rausch S, Metz M, Klotz Ch, Maurer M, Hartmann S. Mast cells orchestrate type 2 immunity to helminths through regulation of tissue-derived cytokines. Proc. Natl. Acad. Sci. USA. 2012;109(17): 6644-6649.
9. Hallgren, J, Gurish MF. Mast cell progenitor trafficking and maturation. Adv. Exp. Med. Biol. 2011;716:14-28.
10. Bowcutt R, Forman R, Glymenaki M, Carding SR, Else KJ, Cruickshank ShM. Heterogeneity across the murine small and large intestine. World J. Gastroenterol. 2014;20(41):15216-15232.
11. Mekori YA, Oh CK, Dastych J, Goff JP, Adachi S, Bianchine PJ, Worobec A, Semere T, Pierce JH. Characterization of a mast cell line that lacks the extracellular domain of membrane c-kit. Immunology. 1997;90(4):518-525.
12. Walczak-Drzewiecka A, Salkowska A, Ratajewski M, Dastych J. Epigenetic regulation of CD34 et HIF1A expression during the differentiation of human mast cells. Immunogenetics. 2013;65(6):429-438.
13. Mays JK. The active management of intrahepatic cholestasis of pregnancy. Curr. Opin. Obstet. Gynecol. 2010;22(2):100-103.
14. Kizjukevich LS. Reaktivnye izmenenija v pochkah pri jeksperimentalnom holestaze [Reactive changes in the kidneys in experimental cholestasis]. Grodno: GrSMU; 2005. 239 p. (Russian).
15. Pirs Je. Gistohimija teoreticheskaja i prikladnaja. Moskow: IL;1962. 962 p.
16. Macjuk JaR. Vozrastnye morfofunktsionalnye izmenenija tkanevyh bazofilov slizistoj zheludka krysjat, rodivshihsja v uslovijah holestaza [Age-related morphofunctional changes in the tissue basophils of the gastric mucosa of rat pups born in conditions of cholestasis]. Zhurnal Grodnenskogo gosudarstvennogo medicinskogo universiteta [Journal of the Grodno State Medical University]. 2010;3:45-48. (Russian).
17. Rebrova OJu. Statisticheskij analiz medicinskih dannyh. Primenenie paketa prikladnyh programm STATISTICA [Statistical analysis of medical data. Application of the STATISTICA software package]. Moskow: MediaSfera; 2003. 312 p. (Russian).
18. Rodrigues СM, Marin JJ, Brites D . Bile acid patterns in meconium are influenced by cholestasis of pregnancy and not altered by ursodeoxycholic acid treatment. Gut. 1999;45(3):446-52.
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