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DEVELOPMENT OF A DOMESTIC ENZYME IMMUNE TEST SYSTEM FOR DETECTION OF ANTI-HEV IGM IN BLOOD SERUM

  • I. S. Zadora Belarusian State Medical University, Minsk, Belarus; Pilot Production of the Institute of Bioorganic Chemistry National Academy of Sciences of Belarus, Minsk, Belarus https://orcid.org/0000-0003-2231-1785
  • S. V. Zhavoronok Belarusian State Medical University, Minsk, Belarus https://orcid.org/0000-0001-9727-1103
  • V. V. Davydov Belarusian State Medical University, Minsk, Belarus https://orcid.org/0000-0002-5672-9509
  • A. S. Babenka Belarusian State Medical University, Minsk, Belarus https://orcid.org/0000-0002-5513-970X
  • L. A. Anisko Belarusian State Medical University, Minsk, Belarus; City Clinical Infectious Diseases Hospital, Minsk, Belarus
  • T. A. Rogacheva Belarusian State Medical University, Minsk, Belarus; City Clinical Infectious Diseases Hospital, Minsk, Belarus
  • V. V. Simirsky Pilot Production of the Institute of Bioorganic Chemistry National Academy of Sciences of Belarus, Minsk, Belarus
  • A. I. Shcherban Pilot Production of the Institute of Bioorganic Chemistry National Academy of Sciences of Belarus, Minsk, Belarus https://orcid.org/0000-0003-1929-0901
  • N. V. Shchuka Pilot Production of the Institute of Bioorganic Chemistry National Academy of Sciences of Belarus, Minsk, Belarus
  • Y. A. Mytko Pilot Production of the Institute of Bioorganic Chemistry National Academy of Sciences of Belarus, Minsk, Belarus
  • G. I. Alatortseva I. I. Mechnikov Research Institute of Vaccines and Sera, Moscow, Russian https://orcid.org/0000-0001-9887-4061
  • L. N. Lukhverchik I. I. Mechnikov Research Institute of Vaccines and Sera, Moscow, Russian https://orcid.org/0000-0002-2997-8892
  • L. N. Nesterenko I. I. Mechnikov Research Institute of Vaccines and Sera, Moscow, Russian https://orcid.org/0000-0002-3825-3906
  • V. V. Zverev I. M. Sechenov First Moscow State Medical University (Sechenov University), Moscow, Russian https://orcid.org/0000-0001-5808-2246
DOI: https://doi.org/10.25298/2616-5546-2023-7-1-57-62
Keywords: hepatitis E virus, enzyme immunoassay, immunoglobulins class М

Abstract

Background. Though the Republic of Belarus does not belong to countries endemic for viral hepatitis E, numerous studies have proved that hepatitis E virus (HEV) actively circulates among humans and animals. However, the circulation is latent that makes it difficult to diagnose the infection in a timely manner. Objective. To develop and evaluate the quality of a domestic ELISA test system for the detection of anti-HEI IgM. Material and methods. 96-well plates, recombinant proteins ORF2 and ORF3 of the 3rd HEV genotype, conjugate of monoclonal antibodies to human IgM with horseradish peroxidase, solutions for dilution of serums and conjugate containing bovine serum albumin. Results. The recommended concentrations of recombinant polypeptides ORF2 and ORF3 of hepatitis E virus genotype 3 for sorption are 2 and 1 μg/ml, respectively. The optimum dilution of the conjugate is 1:13 000. False positive and false negative results were not detected, sensitivity and specificity being at least 99%. The mean value for coefficient of variation within one plate is 6.4%, within two plates is 11.3%. Conclusions. The characteristics of the developed national test system comply with the recommended standards, thus indicating the possibility of its implementation into clinical laboratory diagnostics.

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Peruvian mental health reform: A framework for scaling-up mental health services

PDF Downloads: 191
Published
2023-06-14
How to Cite
1.
Zadora IS, Zhavoronok SV, Davydov VV, Babenka AS, Anisko LA, Rogacheva TA, Simirsky VV, Shcherban AI, Shchuka NV, Mytko YA, Alatortseva GI, Lukhverchik LN, Nesterenko LN, Zverev VV. DEVELOPMENT OF A DOMESTIC ENZYME IMMUNE TEST SYSTEM FOR DETECTION OF ANTI-HEV IGM IN BLOOD SERUM. journalHandG [Internet]. 2023Jun.14 [cited 2024Nov.23];7(1):57-2. Available from: http://hepatogastro.grsmu.by/index.php/journalHandG/article/view/302
Issue
Vol. 7 No. 1 (2023): Hepatology and Gastroenterology
Section
Оригинальные исследования

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